Journal of the Southeastern Association of Fish and Wildlife Agencies

The Journal of the Southeastern Association of Fish and Wildlife Agencies (ISSN 2330-5142) presents papers that cover all aspects of the management and conservation of inland, estuarine, and marine fisheries and wildlife. It aims to provide a forum where fisheries and wildlife managers can find innovative solutions to the problems facing our natural resources in the 21st century. The Journal welcomes manuscripts that cover scientific studies, case studies, and review articles on a wide range of topics of interest and use to fish and wildlife managers, with an emphasis on the southeastern United States.

 

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Abstract: Management agencies within native largemouth bass (LMB; Micropterus nigricans) range across the United States regularly stock non-native pure Florida bass (FLB; Micropterus salmoides) or reciprocal LMB × FLB F1 hybrids to enhance angling opportunities based on public demand. Often, these stocked fish are introduced into waterbodies already occupied by LMB, which readily hybridize with FLB. This hybridization and resulting genetic integration of FLB alleles into the population is often considered the goal of stocking programs. However, managers have little available information to help them determine stocking rates and durations that will allow them to meet management goals associated with the introgression of FLB alleles. To inform such stocking decisions, we created an individual-based model and parameterized it based on the results of a recent FLB stocking program in Lake Ouachita, Arkansas.

Behavioral shifts by bass (Micropterus spp.) anglers toward catch and release practices has severely decreased the ability of managers to control predator-prey dynamics and reduced effectiveness of harvest as a management tool. Lack of harvest reduces managers’ ability to use harvest regulations to influence bass growth and can result in slower growth and poor condition due to excessive predator abundance and reduced prey availability. This scenario appears to have developed in a tropical reservoir where angler harvest has been limited. Largemouth bass (M. nigricans) were experimentally removed from Cerrillos Reservoir, Puerto Rico, to test if targeted population reduction could improve prey availability and condition of largemouth bass in tropical systems.

Crappie (Pomoxis spp.) fisheries are important across much of North America, but most research has focused on management in larger reservoirs. Understanding factors that affect crappie populations in small reservoirs could improve management approaches because manipulation of physicochemical, biological, and morphometric factors may be feasible in these smaller systems. We examined biotic and abiotic features that influence crappie (black crappie P. nigromaculatus and white crappie P. annularis) species dominance, growth, condition, and trap net catch­per­effort (CPE) in 16 small southern U.S. reservoirs (28–357 ha).  Morphometric and physical habitat characteristics, water quality variables, and fish community characteristics were compared to crappie population metrics using an ordination approach.  Reservoirs varied considerably in morphometrics, physi­ cochemical environment, and fish communities, as well as in crappie population dynamics.

The white crappie (Pomoxis annularis) is an important U.S. game fish but is prone to stunting. In Oklahoma, stocking saugeye (Sander vitreus × S. canadensis) is a common management strategy for improving white crappie growth. However, recent work has suggested that saugeye stocking may not be a broadly effective management tool for improving crappie growth rates, especially when controlling for among­-reservoir variation.  Therefore, our objectives were to: (1) determine if stocking of Sander spp. (walleye [S. vitreus], saugeye, or both) improved white crappie growth trajectories along with predicted and observed length­-at­age, and (2) determine if predicted and observed length­at­age yielded similar results to evaluate the potential applicability of using predicted values when observed values are unavailable.

Fisheries

Lynn D. Wright, Timothy J. Bister

1     Examining Hybrid Striped Bass Stocking Rates in Texas Reservoirs: A Trade-off between Abundance and Stocking Efficiency

M. Todd Driscoll, Jacob D. Norman

7    High Mortality of Largemouth Bass Implanted with Transmitters at Colder Water Temperatures

Brent J. Bellinger, Marcos J. De Jesús

14   Hydrilla Management Impacts on a Largemouth Bass Fishery: A Case for a Balanced Management Approach

Daniel L. Bennett, M. Todd Driscoll, Jacob D. Norman

Hybrid striped bass (HSB), which includes palmetto bass (female striped bass Morone saxatilis × male white bass M. chrysops) or its reciprocal sunshine bass (female white bass × male striped bass) support popular fisheries in many Texas reservoirs. Data from 41 reservoirs sampled using gill nets from 1996–2021 (total of 255 reservoir-yr) were used to develop stock-recruit models where fingerling stocking rates were used to predict CPUE of adults in gill nets. Adult relative abundance was described using two size classes based on the statewide 458-mm minimum length limit, catch of fish below (CPUESUB) and above (CPUE458) the limit. A linear mixed-effect model showed stocking rate explained 41–46% of variation in CPUE estimates.  Mean stocking rate from 3–4 yr prior to each gill-net sample were best for predicting recruits for the CPUE458 size class, while stocking rate calculations from years 3–5 and 3–6 explained less variation.

Biotelemetry via surgical implantation of an electronic tag is a common way to examine fish behavior and movement. Previous studies suggest higher post-operative survival should be expected when implanting tags at colder water temperatures. However, during the initial part of our study, all 26 adult largemouth bass (Micropterus salmoides) we implanted with transmitters at water temperatures from 14 to 17 C at Toledo Bend Reservoir, Texas, died within 4 wk post-surgery. To further investigate this phenomenon, we conducted two tagging trials at 13 C, observing post-operative mortality of 100% (n = 5) and 58% (n = 12); all fish that died developed external fungal infections (i.e., saprolegniasis). Post-operative survival was 100% in a third trial at 24 C (n = 6) and no fungal infections were observed. Subsequently, tagging mortality was ≤ 20% when 81 largemouth bass were tagged at water temperatures from 22 to 30 C at Toledo Bend and Lake Fork reservoirs.

Lake Austin, in central Texas, supported a popular trophy largemouth bass (Micropterus salmoides) fishery concomitant with conservative hydrilla (Hydrilla verticillata) management during the period 2002–2011. However, a change from this conservative approach to an aggressive stocking rate of triploid grass carp (Ctenopharyngodon idella) in response to excessive hydrilla growth between 2011–2013 subsequently resulted in the eradication of all submerged aquatic vegetation (SAV). The loss of SAV quickly changed the angling dynamics of the reservoir, resulting in a controversial decline in the quality angling experience. The objectives of this case study were to compare how available population metrics of largemouth bass and important prey fish differed between periods of SAV presence (1997–2013) and absence (2014–2022) to inform which SAV management approach better supports a sustainable trophy fishery goal.

An economic valuation of the recreational sport fishery of Lake Fork in northeast Texas was completed in 2014 and 2015, finding that angler direct expenditures totaled US$18.8 million annually on fishing trips to the reservoir. Although some largemouth bass (Micropterus salmoides) tournament anglers were included in that study, it did not assess economic impacts of six large (>200 participants) tournaments during the study period. Since 2006, largemouth bass tournament effort at Lake Fork has generally increased to comprise half of all fishing activity in the reservoir and is currently believed to account for most of the Lake Fork sport fishery’s economic value. This is despite supporting a renowned trophy fishery for largemouth bass managed by a highly restrictive slot-length limit that makes it difficult to conduct tournaments using traditional formats.

Accurate age estimates are critical in the development, implementation, and assessment of silver carp (Hypophthalmichthys molitrix) management plans. Lapilli otoliths are the most commonly used calcified structures for silver carp age estimation, but studies on the precision of two established preparation methods [i.e., grind-and-burn (GB), thin-section (TS)] are lacking. Therefore, we assessed within-reader, between-reader, and between-method precision for 125 silver carp collected from six rivers throughout the Lower Mississippi River Basin (Arkansas, Cache, Mississippi, St. Francis, White, and Yazoo). Additionally, we compared the effort and material costs associated with each method. Overall, younger ages were estimated with the GB method (median estimated age = 6 yr, range = 3–12) than the TS method (median estimated age = 7 yr, range = 3–13).

Monitoring changes in occupancy (i.e., probability a site has at least one individual of a species) across time is considered an inexpensive alternative to monitoring changes in abundance and can be used to monitor multiple species simultaneously across a watershed. Occupancy can be measured as the proportion of sites where a species is detected during surveys (i.e., naïve occupancy), but is more commonly modeled by surveying sites multiple times to estimate detection probability and address false-positive survey errors (sites that are occupied but with no survey detections of the species). This results in an unbiased estimate of occupancy, but at the expense of more effort. The purpose of this study was to determine management implications of using naïve occupancy versus using modeled occupancy. We generated simulated data to represent monitoring a population, then compared performance of using naïve occupancy vs. modeled occupancy for detecting changes.

Non-native species have sometimes been introduced to increase forage availability and sportfish production, but such introductions have potential for negative as well as positive effects. In 2010, non-native blueback herring (Alosa aestivalis) were found in Lewis Smith Lake, Alabama, due to illegal stocking. Our objective was to quantify food habits and determine potential impacts of blueback herring introduction on body condition and growth of important sportfishes in Lewis Smith Lake. Largemouth bass (Micropterus salmoides), Alabama bass (Micropterus henshalli), and striped bass (Morone saxatilis) were sampled in 2013 and 2014, and diets of these post-blueback herring introduction piscivores were quantified.

Across the U.S., the presence of white-tailed deer (deer; Odocoileus virginianus) in urban areas can create conflicts with residents (e.g., ornamental plant damage). State wildlife agencies approach urban deer management differently from traditional deer management due to diverse community groups, urban stakeholder viewpoints about deer, and other aspects of wildlife management in urban environments. With this variation in mind, we reviewed deer management resources across the U.S. to understand the current state of urban deer management. Of the 46 states with deer populations, 21 had publicly available deer management plans (DMPs; 46%), 22 had only online urban deer management resources available (48%), and three had no urban deer-related information available even though deer were present (7%). Our synthesis revealed that public input was incorporated in all DMPs including input from traditionally under-represented stakeholders.

Wildlife management agencies in regions where chronic wasting disease (CWD) is prevalent have adopted costly management practices to mitigate the spread of this fatal and highly transmissible disease. Non-market valuation represents a critical tool for managers attempting to address these costs, but the mode and methods of contingent valuation (CV) questions can impact valuations due to biases inherent to self-reporting economic decisions. We administered online (n = 1430) and phone (n = 602) surveys in North Carolina and South Carolina to assess what hunters with licenses to hunt white-tailed deer (Odocoileus virginianus) were willing to pay for CWD testing and carcass disposal across survey modes and CV methods. Among the online survey respondents, 34.1% (n = 488) were willing to pay for testing and 43.4% (n = 620) were willing to pay for disposal. From our phone sample, 48.6% (n = 293) were willing to pay for testing and 50.7% (n = 306) for disposal.

Plantings of perennial and biennial forage, such as white clover (Trifolium repens), red clover (Trifolium pratense), and alfalfa (Medicago sativa), commonly are used by managers to increase nutritional resource availability for white-tailed deer (Odocoileus virginianus). Regular mowing and selective herbicide applications are two common practices used to maintain perennial plantings and reduce weed competition. However, there is little information available on how these management activities influence perennial forages or wildlife response. We evaluated the effects of regular mowing on forage production, forage quality, weed coverage, and deer detections as a case study in a perennial forage planting in Tennessee, May–August 2020. We also evaluated deer detections following application of selective herbicides among four fields in Tennessee and North Carolina, October–November 2021.

Many states throughout the range of wild turkeys (Meleagris gallopavo) have delayed their spring wild turkey hunting seasons to allow reproductively active males more time to breed before being harvested and to potentially increase population fecundity rates. Six states in the Southeast recently delayed their spring hunting season by 7 to 14 days. However, there are no published data indicating their previous season frameworks had a deleterious effect on wild turkey reproduction or that delaying the season increased fecundity. In addition to potentially affecting turkey reproduction, changing the season framework may impact hunter behavior (effort and efficiency), success, and satisfaction. Our objective was to see how hunter ef- fort, success, efficiency, and satisfaction changed upon implementing a two-week season delay and a two-week reduction in season length to the spring wild turkey hunting season in south-middle Tennessee.

Understanding hunter satisfaction and behavior under normal and abnormal situations is important for effective management of game species by state wildlife agencies. SARS-CoV-2 (COVID-19) created a global pandemic that coincided with the 2020 spring wild turkey hunting season. Concern was expressed by some wild turkey researchers and biologists that COVID-19 lockdown protocols could result in increased hunting effort and unsustainable harvests because of people having more free time. We assessed how COVID-19 and associated lockdown protocols affected hunter satisfaction and behavior during the spring 2020 wild turkey hunting season by using responses from 2,000 annual surveys of wild turkey hunters (2017–2020) among five focal counties (Bedford, Giles, Lawrence, Maury, and Wayne) in south-central Tennessee.

Nest site selection has a critical effect on nest success for eastern wild turkeys (Meleagris gallopavo silvestris), yet the underlying drivers of nest site selection are often misrepresented in the literature. Early works typically focused on evaluating behavioral ecology of female wild turkeys before nest initiation, under the assumption that female wild turkeys sought out nest sites well in advance of nest initiation. However, recent work has clearly found no evidence of nest site search behavior before the day of nest initiation, thus increasing the need to focus evaluations of resource selection on the day when nest site selection occurs (i.e., the first day of laying). Our objective was to determine if differential selection for landcover characteristics was occurring on the first day of an egg was laid (e.g., date of nest site selection).

The lesser prairie-chicken (Tympanuchus pallidicinctus) has declined precipitously in abundance and currently occupies a substantially reduced portion of its historic range. Within the sand shinnery oak (Quercus havardii) prairies at the southwestern extent of the lesser prairie-chicken’s contemporary range, efforts to conserve the species have been constrained by limited information on how land management practices influence habitat quality, and subsequently, affect lesser prairie-chicken recruitment. From 2008–2011, we captured and radio-tagged hen lesser prairie-chickens to monitor broods during four breeding seasons in western Texas. We evaluated influences of vegetation structure and composition, insect availability, and weather on brood ecology on private lands with continuous cattle grazing but no recent herbicide treatment to control shrubs. We located 32 nests from 50 hens captured.

Land managers in the southeastern United States cultivate rice (Oryza sativa) to provide calorie-dense forage for autumn-migrating and wintering waterfowl and other migratory birds. Conservation planners require accurate yield estimates for rice and other energy-rich croplands to parameterize bioenergetic models and support data-driven, adaptive resource management efforts. We developed a rapid method to efficiently estimate rice yield and quantified associated precision, accuracy, sampling time, and operating costs in the Lower Mississippi Alluvial Valley (n = 16 fields). We compared a visual index of seed-head size and density using ocular scores (1–10; i.e., rapid assessment) to 1-m2 harvested plots within each field. We regressed our visual index against known rice yield estimates (kg [dry] ha–1) and related our yield estimates to rice cultivation practices to inform management actions that maximize yield and cost efficiency for wildlife management.

Monitoring waterfowl populations provides the basis for improving habitat quantity and quality, establishing harvest regulations, and ensuring sustainable waterfowl populations through appropriate management. Waterfowl biologists currently use a variety of population and habitat monitoring methods ranging from informal ground observations to low-level occupied aircraft surveys. Unoccupied aerial systems (UAS) may provide safer and more precise alternatives to traditional aerial survey techniques that are less disturbing to waterfowl, but there is limited information on how waterfowl in winter respond to UAS. Therefore, we compared the behavioral responses of waterfowl to helicopters and UAS on Missouri Department of Conservation

Wild pigs (Sus scrofa) are a highly destructive invasive species and reported to be present in 77% of counties in the southeastern U.S. Wild pigs may negatively affect white-tailed deer (Odocoileus virginianus; hereinafter, deer) and eastern wild turkeys (Meleagris gallopavo silvestris; hereinafter, turkey) via competition over forage or exclusion from preferred areas. To explore effects of wild pigs on spatial distribution of these species within a mixed agriculture-forest landscape, we developed models predicting camera trap detections of deer and turkeys as a function of landcover, calendar season, and wild pig presence. We deployed 147 passive camera traps and collected data for one month during each calendar season during 2020 to 2022 in southwestern Georgia (32,760 camera nights).

Management of wild pigs (Sus scrofa) typically employs some form of population survey methodology, and trail cameras are the most common tool for conducting these surveys. Identification of individual sounders is generally at the foundation of these population surveys. Pelage characteristics and relative age distribution of individuals within the sounder coupled with total sounder size are common characteristics used to identify unique sounders. However, in many populations, the pelage of many wild pigs is either black or wild/grizzled, making pelage characteristics unreliable for sounder identification. Consequently, our objective was to assess the potential of using sounder size and composition as a simple proxy for identification of individual sounders visiting a camera station. Specifically, we aimed to determine the probabilities of encountering two sounders of a specific size and composition at the same camera site.

Animal body mass can be used to estimate age, determine health status, or guide dosage when administering sedatives. Because it can be difficult to weigh live large animals, using morphometric measurements to estimate body mass is sometimes used in field studies. Several statistical models exist for estimating domestic pig mass from morphometric measurements, but models based on domestic animals are likely unreliable estimators of wild pig (Sus scrofa) body mass due to known hybridization between domestic and wild pigs, and variable environmental conditions. The goal of this project was to evaluate several easily obtainable morphometric measurements as predictors of wild pig body mass and compare our estimates with those of models developed from both wild and domestic pigs. We measured neck girth, heart girth, body length, and body mass from 127 wild pigs in Florida and Georgia, and 450 wild pigs in South Carolina.

Grain-based attractants (e.g., corn) are standard among most wild pig (Sus scrofa) trapping and non-invasive sampling efforts (e.g., genetic spatial capture/recapture, camera trapping), but their use is not always feasible due to cost, deployment restrictions (e.g., difficulty of transporting grain into remote areas, property rules), and potential disease concerns associated with concentrating non-target species at bait sites. Attractant deployment and efficacy should be considered by biologists, private landowners, and researchers given the ultimate need to use attractants to attract wild pigs. To examine the efficacy of potential non-grain attractants, we used remote camera grids to identify attractant(s) that maximized wild pig visitation while minimizing non-target species visitation in a forested landscape in the southeastern United States.

Wild pigs (Sus scrofa) are arguably one of the greatest wildlife management challenges facing natural resource professionals and landowners in the U.S., and lethal removal by trapping is often the most cost- and time-effective means for managing populations. Whereas numerous studies have examined the effects of trap type, trap activation designs, and baits on trapping effectiveness, no studies utilizing a conditioning period and accounting for unique individuals/sounders and wild pig social structure have examined the effects of trap door width on wild pig entrance into corral-style traps. Modifying trap door width may impact wild pig entrance rates into corral-style traps with wider doors better facilitating entrance. Our objective was to examine wild pig entry times into standard three-panel corral traps with wooden guillotine trap doors of either 0.8-m or 1.2-m widths.

The gopher tortoise (Gopherus polyphemus) is a species of concern in the southeastern United States, and its distribution is within the range of the longleaf pine (Pinus palustris). One conservation strategy within the state of Alabama has been translocation of adult tortoises to other areas with longleaf pine and sandy soils, including areas outside the current accepted species’ range. Prior examples of such tortoise translocations occurred in two counties in central Alabama: one in the 1960s in Macon County and another in the 1980s in Autauga County. Both introductions occurred near the Coastal Plain fall-line, which is deemed the northernmost landmark designation that tortoises were historically presumed to reside. The status of these translocated tortoise populations had not been recently assessed. Therefore, we surveyed the two locations, captured individuals, and qualitatively examined the minimum known number of alive adult tortoises.

The U.S. Fish and Wildlife Service allows acoustical surveys and automated identification software to determine the presence of the endangered northern long-eared bat (Myotis septentrionalis) and Indiana bat (Myotis sodalis). Analytical software is required to assess presence probability on a site-night basis using a maximum likelihood estimator (MLE) that accounts for interspecific bat misclassification rates. The current standard for occupancy is a returned MLE P-value < 0.05 at the nightly level irrespective of the number of files identified as either northern long-eared bats or Indiana bats. These MLE P-values can vary based on presence of other bat species with similar calls and the relative proportions of all species recorded. Accordingly, there is concern that with few nightly northern long-eared bat or Indiana bat recordings or the presence of large numbers of high-frequency bats, false-negative findings from a swamping effect could result.

Conservation of bats declining from white-nose syndrome (WNS) impacts requires an understanding of both temporal and landscape-level habitat relationships. Traditionally, much of the research on bat ecology has focused on behavior of summer maternity colonies within species’ distribution cores, including that of the endangered northern long-eared bat (Myotis septentrionalis). To further our knowledge of this species, we evaluated multi-season activity patterns in eastern North Carolina and Virginia, including areas where populations were recently discovered. We used passive acoustic monitoring to assess relative and probable activity of northern long-eared bats from October 2016 to August 2021. Northern long-eared bat relative activity was greatest in areas containing greater proportions of woody wetlands and upland pine-dominated evergreen forests.

Understanding the relationships of biotic and abiotic factors to seasonal activity at hibernacula is important for the conservation of bats impacted by white-nose syndrome (WNS). Research on the relative and probable activity patterns of the federally endangered northern long-eared bat (Myotis septentrionalis) primarily has focused on summer maternity colonies, whereas surveys at hibernacula have traditionally relied on external capture and internal counts. We used passive acoustic monitoring to assess the relative and probable activity of northern long-eared bats at 13 hibernacula in western Virginia, from August 2020 to May 2022. Northern long-eared bats were most active near hibernacula during warmer weeks of the fall swarm and spring emergence, when rainfall was low. Similarly, the probability of northern long-eared bat activity was highest near hibernacula during the spring/summer season.

Throughout much of the eastern U.S., many forested ecosystems have lost large amounts of core forest areas due to land-use change, isolating wildlife in forest fragments. The eastern spotted skunk (Spilogale putorius) is considered a species of conservation concern in Virginia, where populations are restricted to spatially disjunct forest patches in the central Appalachian Mountains. We caught and radio-tagged eastern spotted skunks in the Appalachian Mountains of Virginia to assess whether current habitat fragmentation restricts skunk movements and hence distribution, potentially leading to isolation among habitat patches. Denning home range size (approximately 3.7 km2) in our study was smaller than those in other studies of eastern spotted skunks and excursive movements were primarily limited to core forested areas (>2 km2). Core forested areas were used more than non-forested and forest-edge areas.

Southern fox squirrels (Sciurus niger niger) have been declining due to habitat fragmentation, cover type conversion, and fire suppression in the Southeast. A decrease in growing season burns has led to hardwood encroachment and forest mesophication that benefit the competing eastern gray squirrels (S. carolinensis). In the southern Coastal Plain and Piedmont of Virginia, these pattern raises the question of whether gray squirrels are competitively excluding southern fox squirrels in these altered landscapes. From October 2019 to October 2020, we conducted continual camera trapping for southern fox squirrels and gray squirrels on the Big Woods/Piney Grove Complex (BWPGC) and at Fort Barfoot (FB) in the Coastal Plain and lower Piedmont of Virginia, respectively. Both sites are among the few areas that still contain large, intact pine savanna and mixed-pine hardwood forests in southeastern Virginia.

Fisheries

Graham F. Montague, Richard A. Snow, Douglas L. Zentner, Austin D. Griffin

1    Comparing Precision of Otolith and Pectoral Spine Age Assessments for Black and Yellow Bullheads

Joseph V. Siegel, Stuart Welsh, Nate Taylor, Quinton Phelps

10   Size Structure, Age, Growth, and Mortality of Flathead Catfish in the Robert C. Byrd Pool of the Ohio and Kanawha Rivers

Steven J. Rider, Travis R. Powell

17    Characteristics of Commercial Paddlefish Harvest from a Provisional Fishery in the Alabama River, Alabama

John Davidson, Clayton Raines, Curtis Crouse, Christopher Good, Brandon Keplinger

27    Evaluating Brook Trout Egg and Alevin Survival at Different Temperatures in Simulated Karst Environments with Marl Sedimentation

Despite the broad geographical range of bullhead catfishes (Ameiurus spp.), their population vital rates have rarely been studied. Estimation of vital rates requires accurate age estimates and otoliths generally are considered to be the most accurate and precise aging structure for most fish species. However, pectoral spines of some ictalurid species have been used to generate precise age estimates of younger fish. Although previous studies have compared age estimates between otoliths and spines for large-bodied, longer-lived catfishes, there have been few comparable studies for bullheads. Our objectives were to compare the reader precision and preparation times of lapilli otoliths and pectoral spines (articular process [AP] and basal recess [BR] sections) to determine which aging structure is most precise and efficient for age analysis of black bullhead (Ameiurus melas) and yellow bullhead (A. natalis).

Flathead catfish (Pylodictis olivaris) were sampled in the Robert C. Byrd Pool of the Ohio and Kanawha rivers, West Virginia, to inform management decisions based on population characteristics of size structure, age, growth, and mortality. Sampling was conducted with low-frequency boat electrofishing during late May to early June over a four-year period (2017–2020). We examined size structure using proportional size distribution indi- ces. Growth was evaluated using otolith-derived ages, a von Bertalanffy growth curve, and mean length at age data, including comparisons to published mean length at age data of other populations. Annual mortality was estimated with a weighted catch curve. We documented a high-density population (mean CPUE = 49 fish h–1) with low mortality (A = 11.8%), characterized by slow growing individuals with a maximum recorded age of 36.

Due to overharvest of paddlefish (Polyodon spathula) throughout Alabama, the Alabama Division of Wildlife and Freshwater Fisheries (ADWFF) approved a moratorium prohibiting the recreational and commercial catch, possession, and harvest of paddlefish beginning November 1988. However, due to increased demand for paddlefish eggs, a provisional fishery for commercial paddlefish harvest was approved beginning March 2013 in the Alabama River, Mobile River Basin, Alabama. As part of this provisional fishery, a new reporting form was required of all commercial paddlefish harvesters to record their daily harvest and effort. We summarized and quantified commercial paddlefish harvest and harvester data from these reports to examine spatial and temporal harvest patterns from 2013 to 2017 and compare to data collected by ADWFF biologists in 2016. A total of 4861 female paddlefish were harvested in all commercial years combined.

Brook trout (Salvelinus fontinalis) have been extirpated from many karst-geology streams in West Virginia; however, the causes are not fully understood. Specifically, the impact of calcareous precipitate (marl), which is common in hard-water environments, has not been evaluated as an im- pediment to juvenile survival. Accordingly, two lab-based studies were conducted to determine if brook trout egg and alevin survival is inhibited by marl. In the first study, three aeration treatments were applied to water from a limestone spring source (13–14 C; ~300 mg L–1 hardness), resulting in different pH levels and an increasing degree of marl precipitate. Treatments included raw/untreated (RU; no marl), once-aerated (OA; limited marl), and continuously aerated (CA; significant marl) water. Brook trout eggs obtained from a local hatchery were fertilized and stocked among gravel-filled trays receiving each water type.

Understanding walleye (Sander vitreus) spawning behavior is important for managing walleye fisheries, but such information is limited for Appalachian reservoirs. We assessed spawning movements and spawning locations for a reestablished walleye population in Cheat Lake, West Virginia. We tagged fifty-two walleye with acoustic telemetry transmitters to evaluate environmental correlates associated with pre-spawn movements and to deter- mine spawning locations. Using an information-theoretic approach, we compared candidate logistic regression models to determine which environmental variables best explained upstream movements to spawning areas. The two models with the most support both included additive effects of year and water temperature, with sex also included in the second of these models. Water temperature had a significant positive relationship with pre-spawn movements in each model.

Catching a state record fish is a significant accomplishment in the life of any angler. The need to have a state agency biologist present to verify the record fish can delay the certification of the fish, possibly leading to changes in the fish’s weight. Few published studies have directly investigated the impact of preservation method on weight change of fish following capture. We examined four fish species: black crappie (Pomoxis nigromacula- tus), bluegill (Lepomis macrochirus), flathead catfish (Pylodictis olivaris), and largemouth bass (Micropterus salmoides) to identify the best preservation method for minimizing change in weight post-catch. We evaluated four preservation methods including holding fish alive, on ice, in an ice bath, and in a freezer for either 24 or 48 h.

Stream fishing for black bass (Micropterus spp.) is a popular outdoor recreational activity in northern Arkansas. After construction of a new access area on Crooked Creek, Arkansas in 2017, anglers expressed concerns about increased fishing pressure and possible overharvest of smallmouth bass (Micropterus dolomieu). In 2019, we conducted a creel survey (60 sample days over six months) at five public accesses, including the new access, on a 35-km section of Crooked Creek. We also tagged 195 fish in an associated one-year exploitation study to address requests for stricter regulations and mandatory catch and release for smallmouth bass. Estimates of fishing pressure (20,521 h) and smallmouth bass catch rates (1.13 fish h–1) were both high. We saw high tag reporting rates (61%) during the first two months of the exploitation study.

Declining angler harvest rates of largemouth bass (Micropterus salmoides) have increasingly led to small impoundments containing over-crowded largemouth bass populations. Various methods to correct or prevent crowded largemouth bass populations have been used by fisheries man- agers, with mixed results. We removed largemouth bass from two small impoundments in South Carolina using boat electrofishing over two consecu- tive years, with targets of removing 40–50% of the largemouth bass populations each year. We used relative weight (Wr) as the removal criterion, such that all largemouth bass displaying condition Wr < 95 were removed. Largemouth bass population sizes were estimated using mark-recapture in each impoundment for large (≥200 mm TL) and small (<200 mm TL) largemouth bass length groups.

Negative impacts from non-native congener introductions have emerged as an immediate threat to black bass conservation and management. Largemouth bass (Micropterus salmoides) historically comprised the sole black bass fishery in Moss Lake, North Carolina. Alabama bass (Micropterus henshalli) were illegally introduced into Moss Lake and were first detected during a 2008 electrofishing survey conducted by North Carolina Wildlife Resources Commission biologists. Since this detection, Alabama bass rapidly increased in abundance throughout the reservoir, while largemouth bass abundance declined concomitantly and reached a low equilibrium, except within cove habitat of the upper reservoir. Alabama bass CPUE was generally 2–3 times higher than largemouth bass CPUE during the study, but Alabama bass were overall smaller in size and in poorer condition than largemouth bass.

The largemouth bass (Micropterus salmoides) population in South Mill Creek Lake, a centrarchid-dominated, eutrophic small impoundment in West Virginia, was managed under a 305-mm minimum-length limit until 2007. Under this regulation the population was typified by consistently low proportional size distribution (PSD) values, low quality-length CPUE, excessive juvenile recruitment, and poor length structure. Therefore, in 2007 a protected slot limit (PSL) regulation (305–406 mm) was implemented to shift the size structure of the fishery. Spring (May) boat electrofishing surveys were conducted pre- and post-regulation (2003–2022) to evaluate fishery response under both regulatory regimes. The largemouth bass population of a similar system, Kimsey Run Lake, was sampled using the same methods over the same time periods.

Production of submersed aquatic vegetation (SAV) is promoted for waterfowl forage through hydrological management in brackish tidal impoundments along the south Atlantic coast, USA. This management also promotes production of aquatic invertebrates as food resources for many bird species. We conducted a field experiment to compare effects of traditional complete drawdown to fissure substrates versus a novel partial drawdown (i.e., mudflat to 10 cm depth) on aquatic invertebrate biomass in impounded and non-impounded tidal wetlands in the Ashepoo, Combahee, and Ed- isto Rivers (ACE) Basin, South Carolina. We sampled 20 randomly selected impoundments (complete drawdown, n = 8; partial drawdown, n = 12) and adjacent non-impounded tidal marsh across three properties in August 2016, November 2016, January 2017, and April 2017.

We analyzed a three-year consolidated sample of Louisiana hunters’ responses to the Louisiana Game Harvest Surveys (LAGHS) distributed via email and mail in May following the 2016–2017, 2017–2018, and 2018–2019 hunting seasons. To determine whether the distribution modes pro- duced different results, both modes asked identical questions about hunting effort, harvest, and age. We used generalized linear mixed models to test hypotheses about hunters’ days hunted, harvest, representation of age classes, and effect of age-weighting (i.e., weighting responses based on the differ- ence in proportion between individual age classes in the response sample and the original license population) across survey modes. We compared days spent hunting and species harvested across distribution modes. We received 42,346 qualified email responses with a qualified response rate of 19.3%, and 6387 qualified mail responses with a qualified response rate of 14.1%.

Since the spread of white-nose syndrome in North America, several bat species have shown precipitous declines in abundance and distribution. With lower netting detection probabilities for the currently threatened but proposed endangered northern long-eared bat (Myotis septentrionalis) and endangered Indiana bats (Myotis sodalis), determination of presence or absence for regulatory clearance often has shifted to the use of acoustic sur- veys. However, acoustic surveys are unable to differentiate between non-reproductive individuals versus a maternity colony. We used recorded nightly echolocation pass counts of bat species-specific probabilities with maximum likelihood estimator (MLE) scores to determine thresholds by cover type and reproductive period whereby the potential for northern long-eared bat or Indiana bat maternity colonies occurs. Where nightly MLE P-values were

The gray bat (Myotis grisescens) is a cave-obligate species that has been listed as federally endangered since 1976, following population declines from human disturbance at hibernation and maternity caves. However, with cave protection, most gray bat populations have increased. As part of a project examining bat use of transportation structures as day-roosts, we continuously acoustically monitored 12 riparian sites within the Clinch River Watershed of southwest Virginia from March through November, 2018–2020. We used 15 different landscape and weather-related variables in gener- alized linear mixed models to determine factors influencing gray bat presence and activity. Seasonal activity patterns were similar among years, but the number of nightly gray bat calls increased with each passing year, consistent with positive population trends observed at winter hibernacula.

Species distribution models enable resource managers to avoid and mitigate impacts to, or enhance habitat of, target species at the landscape level. Persistent declines of northern long-eared bats (Myotis septentrionalis) due to white-nose syndrome have made acquisition of contemporary data difficult. Therefore, use of legacy data may be necessary for creation of species distribution models. We used historical roost and capture records, both individually and in combination, to assess the distribution and availability of northern long-eared bat habitat across the 670,000-ha Monongahela Na- tional Forest (MNF), West Virginia, USA. We created random forest presence/pseudo-absence models to examine influences of various biotic and abi- otic predictors on both roosting and foraging presence locations of northern long-eared bats. Predicted northern long-eared bat habitat was abundant (43.1% of the MNF) and widely dispersed.

Hierarchical conservation and management of Indiana bat (Myotis sodalis) habitat may benefit from use of species distribution models. White-nose syndrome has caused additional declines for this endangered bat, requiring use of historical presence locations for habitat-related analy- ses. We created random forest presence/pseudo-absence models to assess the distribution and availability of Indiana bat habitat across the 670,000-ha Monongahela National Forest (MNF), West Virginia, USA. We collated historical roost and capture locations, both individually and in combination, to examine impacts of various biotic and abiotic predictors on roosting and foraging habitat of Indiana bats. Our final concordance map suggests that In- diana bat habitat was abundant (37.2% of the MNF) but localized, with predicted suitable areas often associated with edges of dry-calcareous forests.

Achieving a target population size is often the first goal of species restorations. From 2012 to 2014, the Virginia Department of Wildlife Resources released 75 elk (Cervus canadensis) originating from Kentucky into Buchanan County in southwestern Virginia. These individuals were ear tagged with unique numbers upon release with an additional 33 elk tagged within the Virginia Elk Management Zone (VEMZ) from 2019 through early 2022. To assess post-release population size, we conducted visual driving surveys throughout Buchanan County from January through mid-April, 2021 and January through March, 2022, counting elk and noting sex, age class, and tagged individuals when observed. We conducted four surveys an- nually, each consisting of pooled elk counts from eight driving routes, and calculated a Lincoln-Petersen population estimate with Chapman’s bias cor- rection for each survey, then averaged estimates for each year.