Annulus Validation, Time of Formation, and Mean Length at Age of Three Sunfish Species in North Central Florida
The visible opaque bands in transverse sections of sagittal otoliths from bluegill (Lepomis macrochirus), redear sunfish (L. microlophus), and redbreast sunfish (L. auritus) were verified as annual rings. The verification was done using intraperitoneal injections of oxytetracycline followed by otolith examination after the fish were at liberty for 1 year in a north central Florida pond. Injections of 75 mg/kg wet body weight resulted in the best rate of fluorescent ring formation. Saggital otoliths from bluegill and redear sunfish populations in Newnans Lake Florida were sampled monthly to...
