William D. Harvey

During a 2-year period, 250,000 Florida largemouth bass fingerlings were stocked among the endemic northern largemouth bass population of Tradinghouse Creek Reservoir (1985 = 185/ha, 1986 = 123/ha). We used electrophoresis to estimate proportions of Florida, northern, and Florida x northern intergrade largemouth bass within pre-stocking, stocking-year, and post-stocking year cohorts. Ages of largemouth bass were determined by examination of whole otoliths. The proportion of Florida phenotypes within the stocking-year cohort was not increased when the stocking rate was more than 50/ha...

The 4 North American species of the genus Morone were evaluated using isoelectric focusing for determination of species specific protein phenotypes. Each species could be characterized by a pair of protein bands that had isoelectric points in the 3.0 to 5.0 pH range. These diagnostic protein bands were then used to successfully identify 3 congeneric hybrids. The technique of isoelectric focusing yields results that are accurate in determination of species within this genus and serves as a powerful complement to other electrophoretic techniques in analyses of Morone populations.

As a part of a cooperative effort between the Fisheries and Law Enforcement Division of the Texas Parks and Wildlife Department, a comprehensive program for forensic identification of fish and wildlife species has been implemented. Results of comparisons of blood stains from various wildlife species indicate that isoelectric focusing of blood produces genetic "fingerprints" that are characteristic for individual species. Results of 3 representative cases are reported and the program enactment is outlined.