Identification of Gulf of Mexico Sciaenids by Isoelectric Focusing
Sarcoplasmic proteins were isolated from skeletal muscle of 14 species of Gulf of Mexico sciaenids encompassing 11 genera utilizing isoelectric focusing (lEF). Individuals from the 11 genera were distinguishable. However, intrageneric comparisons (Cynoscion and Menticirrhus) were constrained by similar protein banding among congenerics and required a high resolution pH gradient (pH 4-5) to produce species-specific patterns. A graphical representation of differences in banding patterns among the 14 species was provided by densitometric tracings. Isoelectric focusing provided qualitative...
