Removal trapping was used to study opossum (Didelphis virginiana) demography at an upland site in western Tennessee during March 1983. Monthly differences in scent-station visitation were assessed at 5 western Tennessee localities, representing upland and lowland habitats, from April 1982 to May 1983. A density of 1 opossum/ 15.6 ha was determined using removal trapping. Yearlings made up 64% of the 14 animals captured. Adult and pouch-young sex ratios were approximately 100:100. Mean litter size was 8.8 young. Monthly differences in scent-station visitation were found in lowland habitat (P = 0.02) but not in upland habitat (P = 0.38).