Journal of the Southeastern Association of Fish and Wildlife Agencies

The Journal of the Southeastern Association of Fish and Wildlife Agencies (ISSN 2330-5142) presents papers that cover all aspects of the management and conservation of inland, estuarine, and marine fisheries and wildlife. It aims to provide a forum where fisheries and wildlife managers can find innovative solutions to the problems facing our natural resources in the 21st century. The Journal welcomes manuscripts that cover scientific studies, case studies, and review articles on a wide range of topics of interest and use to fish and wildlife managers, with an emphasis on the southeastern United States.

 

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Sustainability of eastern wild turkey (Meleagris gallopavo silvestris, hereafter turkey) populations following translocation is dependent on reproductive success. Extensive efforts to restore turkeys to east Texas using translocation have yielded mixed results, leading to low-density, fragmented populations. Dynamics of a translocated turkey population are dependent on the outcome of nesting activity and nest success which can be influenced by vegetative characteristics selected by females when nesting. Because translocated turkeys transition from natal to new habitats, understanding patterns of nesting activity and vegetative characteristics selected by nesting females are important to continued restoration of turkey populations. We translocated 78 female and 23 male turkeys from Iowa, Missouri, and West Virginia to southern Angelina National Forest near Zavalla, Texas, during 2016?2017.

Historically, Rio Grande wild turkeys (Meleagris gallopavo intermedia) in south central Texas have been at lower densities than in other por-tions of the state. Within the Oak-Prairie Wildlife District of Texas, Rio Grande wild turkey regulatory restrictions are different for counties in the east- ern and western portions of the region. Due to perceived increases in turkey density in the eastern portion of the ecoregion, Texas Parks and Wildlife Department (TPWD) considered increasing the bag limit in the Rio Grande wild turkey spring-only 1-bird zone counties to increase hunting oppor-tunities. However, if regulatory changes are to be considered in the absence of estimates of abundance and harvest rate, then estimates of demograph- ic parameters will provide the basis for regulatory decision-making.

The mourning dove (Zenaida macroura; hereafter dove) is among the most iconic symbols for hunting in the southeastern United States. Con-servation and management of this species is a priority for many state wildlife management agencies. Annual banding efforts are one of the main meth-ods used to measure survival and recovery rates, which aid in harvest management recommendations. We examined a number of dove captures using five different bait types over a two-year period in southwestern Louisiana and performed a banding cost analysis. We found milo to be the most efficient bait for banding new doves and total captures (newly banded doves and all recaptures combined), followed by browntop millet. Similarly, milo was least expensive bait per volume, most economical per capture, and yielded the most captures, with a cost of US$1.09 for each new band deployed and $0.25 per capture.

Mottled ducks are typically geographically separated into two sub-species: peninsular Florida (Anas fulvigula fulvigula) and the western Gulf Coastal (WGC) (A. f. maculosa). Between 1975 and 1983, >1,200 mottled ducks were introduced to coastal South Carolina primarily from the WGC range. A late summer banding program was initiated in 2008 within the Santee Delta and the Ashepoo, Combahee, and Edisto Rivers Basin in South Carolina to estimate mottled duck survival and harvest probability. We acquired 3,594 banding and 525 recovery records of mottled ducks banded between 2008-2018. We used the dead recovery model with Brownie parametrization in Program MARK to estimate annual survival (S) and recovery probabilities (f) among combinations of age, sex, year, and band material (aluminum or stainless steel). Annual survival was greatest for adult males (0.60 ± 0.03 [SE]), followed by adult females (0.57 ± 0.04), juvenile females (0.44 ± 0.13), and juvenile males (0.32 ± 0.07).

Species richness, abundance, and genetic variability often decrease in bird populations when their habitats are subjected to anthropogenic activity. Regular and early monitoring of genetic diversity can give researchers and wildlife managers insight into the genetic health of populations so that action can be taken before inbreeding, loss of disease resistance, and population declines occur. We measured genetic diversity in populations of avian species that are increasingly exposed to anthropogenic changes. We analyzed samples from 89 individual birds from three locations in Gwinnett County, Georgia. Samples were collected from a total of seven species, four migratory [myrtle warbler (Setophaga coronata), American robin (Turdus migratorius), American goldfinch (Spinus tristis), and field sparrow (Spizella pusilla)] and three non-migratory [northern cardinal (Cardinalis cardinalis), brown-headed nuthatch (Sitta pusilla), and white-breasted nuthatch (S. carolinensis)].

Environmental and anthropogenic stimuli can impact a variety of species' behavioral ecology. White-tailed deer (Odocoileus virginianus) re- spond both spatially and temporally to various types of disturbance; however, our understanding of how disturbance impacts deer behavior is typically regulated to studies where white-tailed deer are the targeted species. We used GPS data collected from female white-tailed deer (n=10) to evaluate space use in response to small game hunting activities based on whether an individual was within the hunted area (actively disturbed) or outside (passively disturbed). We found that deer movements per 20-minute period did not differ between actively (59 m, SD=26.21) and passively (57 m, SD = 52.82) disturbed individuals. We also found no difference in home range (99% utilization distributions) or core range (50% utilization distribu- tions) size between actively and passively disturbed individuals.

Hunting white-tailed deer (Odocoileus virginianus) with dogs (herein, dog-deer hunting) has been steeped in tradition and controversy. Today in the United States, dog-deer hunting for white-tailed deer only occurs in nine states of the Southeast. We reviewed hunting regulations and primary literature, interviewed state-agency biologists, and simulated deer movements on national forests to investigate the current status of dog-deer hunting and develop recommendations for best practices to manage methods associated with the tradition. We recommend: 1) developing plans for consistent communication among agencies and stakeholders, 2) allowing dog-deer hunting where the practice is accepted culturally, 3) developing and enforcing permit systems to ensure hunter accountability, and 4) encouraging or requiring tracking and correction collars on dogs to reduce trespass.

Wild pig (Sus scrofa) populations have exploded across much of the southeastern United States. In order to combat increasing wild pig num- bers in an effort to reduce both ecological and economic damage caused by wild pigs, toxicant baits are being investigated as a possible method to reduce wild pig numbers at the local scale. In fall 2017, we tested the HogStopper? feeder to ascertain if this feeder design would deliver bait to wild pigs while preventing non-target species from accessing bait. We examined visitation rates at feeders for wild pigs and non-target species using both digital and video cameras. We had a three-week acclimation period (feeder doors remained open allowing free access to bait) followed by a three-week activation period (feeders were closed). Wild pigs visited eight of 10 feeders but fed from only one of these feeders during the activation period.

Te Central Georgia Bear Population (CGBP) is of special conservation concern due to its relatively small population size and isolation from other bear populations in the southeastern United States. Plans to widen Georgia State Route (SR) 96, which bisects the CGBP, have potential to negatively impact the population. Highway underpasses are being planned to mitigate these impacts. During 2012-2015, we captured and ftted 63 American black bears (Ursus americanus) with global-positioning-system collars and used remote, infrared cameras to document bear crossings along SR 96. We evaluated landscape characteristics associated with 212 bear crossings (210 documented via global-positioning-system collars, two with cameras) using a resource selection function approach and generalized linear mixed-models. We noted that bears were more likely to cross SR 96 where the highway bisected upland habitats.

Recruitment and retention of future conservationists are key issues for many natural resources agencies and organizations. Engaging chil-

We conducted a survey to evaluate 2003-2008 sales of private rural lands in Mississippi that were purchased for wildlife-related recreational

uses. Land parcels purchased (n=800; totaling 102,611 ha) were predominately forested (45%), followed by agricultural lands (26%), early successional habitats and recently planted pine forests (25%), and other lands (>3%). Hunting (99%) and motorized vehicle use (65%) were common uses reported on properties. Wildlife-related recreation accounted for an estimated 34% (US$1,566/ha) of overall value of rural lands sold. Cover types such as forests, and amenities, such as lodging and roads, increased potential recreational use and prices paid for rural properties. We speculate that demand and prices

paid for managed, high-quality properties that support wildlife populations for recreational use will increase with time. To better estimate value of rural

Each year the number of recreational visitors to southeastern national forests increases which brings new challenges for wildlife managers related to visitor activity and their potential effects of visitors on natural resources. This increasing visitation and recreation may affect species inhab-iting streams if these habitats are modified by visitors. North Carolina includes some of the last stable populations of a fully aquatic salamander, the eastern hellbender (Cryptobranchus alleganiensis alleganiensis). Few studies have assessed instream recreation (e.g., fishing, tubing, swimming) and

Aeration can circulate waters by disrupting thermal density differences associated with stratification, allowing homogenization of tempera- ture, oxygen, and other physicochemical characteristics within the water body. Use of lake and pond destratification as a management tool has been increasing in recent years, yet data are limited regarding its effects on fish communities. This case study examines the response of a largemouth bass (Micropterus salmoides) population to destratification in a 2.4-ha pond over nearly a decade. Biomass (35.8–42.8 kg ha–1) and density (51–93 fish ha–1) of stock-sized (≥200 mm TL) largemouth bass were consistently low prior to installation of the system; however, biomass tripled (129.8 kg ha–1) and density quadrupled (334 fish ha–1) 3.5 years after system initiation and remained high for the duration of the study.

We described sociodemographics and expenditures of black bass (Micropterus spp.) anglers participating in eight different tournament types on Lake Guntersville, Alabama, in 2013. We estimated 9035 anglers fished in 259 tournaments. Most anglers were middle- to older-age Caucasian males with an annual household income of over US$75,000, and who had participated in tournaments for over 15 years. Travel distance, expenditures, non-Caucasian participants, residence location, number of times fishing on Lake Guntersville, entry fees, and club membership all differed among tournament types. Anglers spent $4.5 million (average of about $500 per tournament per angler) that generated $208,000 in state and local tax revenue over a one-year period. However, expenditures varied over an order of magnitude among different tournament types.

The Mobile-Tensaw Delta is an 8231-ha oligohaline, tidal estuary that supports a popular largemouth bass (Micropterus salmoides) fishery. This system is productive, with an abundant bass population and above-average recruitment to age-1. But recruitment of the 2004 year-class was poor post-Hurricane Ivan, prompting angler concerns about the population. We considered improvements in the fishery were most likely achieved by stock- ing advanced-size fingerlings. Larger, older fish reared on live food were expected to provide a competitive advantage over native fish and exhibit higher survival than stocking smaller fingerlings. As this is an expensive venture, we tested this proposal in two tidal watersheds, Byrnes Lake and Threemile Creek, and a freshwater control, Monroe County Lake. During 2010–2014, a total of 16,380 advanced-size (97–354 mm TL) largemouth bass were tagged and stocked at approximately 10 months old.

Public fishing areas (PFAs) in Georgia are intensively managed freshwater impoundments that provide a variety of fishing opportunities to an- glers. Management efforts and fishing regulations at these PFAs depend on understanding basic aspects of recreational fishing pressure, catch, and har- vest. Accordingly, we conducted a roving creel survey during January–December 2013 at Marben PFA in middle Georgia to quantify sport fishing total effort, catch, harvest, and fish catch by species, number, and weight in 14 lakes. Almost all of the anglers interviewed (84% of the 1159 parties) targeted a preferred species; of these anglers, 34.7% targeted a second species, and 5.7% targeted a third species. Sunfish (Lepomis spp.) ranked highest among pri- mary, secondary, and tertiary targeted species, whereas channel catfish (Ictalurus punctatus) was the highest ranked quaternary targeted species.

Pond enhancements such as adding pelleted feed or stocking threadfin shad (Dorosoma petenense) are sometimes used in the management of pond fisheries, but their relative impacts on growth and reproduction at multiple levels of the food web are not often fully evaluated. We used stable isotope analysis to indicate the contribution of pelleted feed to bluegill (Lepomis macrochirus) reproduction and growth, and ultimately to largemouth bass (Micropterus salmoides) growth in the presence and absence of threadfin shad via two different approaches: a pond experiment and sampling of established ponds. Bluegill growth and reproductive metrics increased with increased rates of pelleted feed provided. Bluegill nitrogen signature decreased with pelleted feed in the small pond experiment, suggesting feeding at a lower trophic level with increased feed. Largemouth bass nitrogen signature results showed similar trends to that of bluegill, although not statistically significant.

Population-level studies often require age estimation of fish, but populations in small rivers and streams are generally smaller than those in large rivers or reservoirs. Therefore, non-lethal aging methods are generally recommended to minimize the potentially negative effects of sampling on population size. Accordingly, our main goal was to compare otoliths and scales as structures for estimating the age of redbreast sunfish (Lepomis auri- tus) and green sunfish (L. cyanellus) in an urban watershed. Reader agreement was greater for otoliths (88%–89%) than for scales (73%–79%), and pre- cision (mean CV) in age estimates was better for otoliths (3.7%–4.0%) than scales (6.1%–9.4%) for both species. Readers were significantly more confi- dent in their otolith-derived age estimates than scale-derived age estimates for both species. For redbreast sunfish and green sunfish, age estimation bias between readers was apparent for scales, but not for otoliths.

Telemetry-based study of alligator gar (Atractosteus spatula) movement in the lower Trinity River, Texas, indicated that fish primarily re- mained within discrete home ranges less than 60 river kilometers (rkm), supporting the potential for local-scale management. However, the temporal scale of inference was limited (22 months), which may inadequately represent fish movements and home range size at the lifetime (i.e., ≥50 years) scale. Therefore, we used otolith microchemistry to examine the long-term movements of alligator gar (n = 59; total length range 1152 to 2420 mm, age range 4 to 60 years) between the lower Trinity River and Trinity-Galveston Bay system. Strontium:calcium (Sr:Ca) concentrations were measured along laser-ablated transects from the otolith core (i.e., time at hatch) to the edge (i.e., time at capture) for fish collected throughout the system, document- ing movements between the river (freshwater) and bay (saltwater).

This study evaluates the production and economic feasibility of a fixed-floor, in-pond raceway system (IPRS) to supply processor and niche live catfish markets while also highlighting production issues that arose by targeting these two markets. A west Alabama catfish producer grew hybrid catfish (♀ Ictalurus punctatus x ♂ Ictalurus furcatus) to market size in two production cycles (2012–2013 and 2013–2015). Management and harvest of IPRS-raised catfish changed from production cycle 1 to production cycle 2 due to higher market prices received from niche-live fish market buyers. The high density of fish in the IPRS and the small size of the raceway cells made it easier to frequently harvest small quantities of catfish for niche markets. Small quantities of catfish cannot be harvested efficiently from the large ponds traditionally used in the U.S. farm-raised catfish industry.

Stream-dwelling salmonid populations may be affected by both density-dependent and density-independent processes, but the relative im- portance of each may vary both spatially and temporally. We quantified population fluctuations of two unexploited rainbow trout (Oncorhynchus my- kiss) populations in western North Carolina over a 10-year period and examined the effects of spring discharge and adult abundance on recruitment. Both rainbow trout populations exhibited high degrees of temporal variability in density during the study. High spring flows that occurred during the incubation and emergence periods of rainbow trout were associated with densities of age-0 and age-1 trout in both streams. Age-0 densities were also associated with adult densities in one stream but with no evidence of a density dependent relationship. These results suggested that fluctuations in rain- bow trout density in these two streams were determined primarily by a density-independent factor.

Brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) were stocked at two sizes, small (approximately 254 mm TL) and large (approximately 356 mm TL), in Apalachia Reservoir, North Carolina, to determine the best size and species to create a trophy put-grow-and-take fish- ery. Trout were tagged and stocked in December 2012–2015 and collected with annual boat electrofishing and gill-net surveys. Small trout of both spe- cies grew faster in length than large trout; however, brown trout of both size classes reached larger sizes (≥500 mm TL). Large brown trout were highly piscivorous throughout the study, whereas small trout of both species fed primarily on macroinvertebrates within the first four months in the reservoir and converted to a more piscivorous diet by 16 months when they had reached a mean TL of ≥400 mm. Large rainbow consumed mainly macroinver- tebrates until becoming more piscivorous after 16 months in the reservoir.

Florida freshwater habitats provide many essential functions including flood control and nutrient sequestration. While serving as habitat for many fish and wildlife species, wetlands also contribute significantly to the outdoor recreation industry. Despite these services, aquatic habitats contin- ue to face threats, such as urban encroachment, water withdrawals, water-level stabilization, sedimentation, non-native species introduction, cultural eutrophication, and climate change. With Florida’s increasing human population, encroachment and development continues into natural areas, stress- ing aquatic habitats and the fish and wildlife that depend on healthy wetlands. This paper summarizes a GIS process for identifying publicly accessible freshwater resources and presents a multi-criteria decision analysis tool for prioritizing those resources to guide management considerations.

The Arkansas Game and Fish Commission (AGFC) budgeted approximately $US250,000 for air operations in fiscal year 2017, 74% of which was for aerial observation by manned aircraft. Small unmanned aircraft (sUAS) have lower operating costs than manned aircraft, and thus significant cost savings could be experienced were sUAS to replace manned aircraft. However, it is first necessary to evaluate that data from sUAS are comparable to data from manned aircraft. Therefore, angler pressure counts were conducted simultaneously using both manned aircraft and sUAS within the four management zones of Beaver Dam Tailwater, Arkansas. Counts of boats, boat anglers, boat occupants, and non-boat anglers were compared between methods using a Wilcoxon paired signed rank test. More boat anglers were recorded using the manned aircraft than the sUAS (v = 55, P < 0.01); observ- ers in the manned aircraft appeared to have recorded some non-fishing boat occupants as anglers.

The Arkansas Game and Fish Commission (AGFC) conducted 56 h of unmanned aircraft operations between February 2017 and April 2018. Operations involved video and still photography for both scientific and public outreach purposes, mapping, and live surveillance. Some operations were conducted under the small unmanned aircraft rule (Title 14 Code of Federal Regulations §107) established by the Federal Aviation Administra- tion (FAA), while some were conducted under the terms of a Certificate of Authorization from the FAA. The initial training program consisted of a 32-h in-person class. After gaining operational experience, subsequent training expanded to a 112-h class with greater emphasis on hands-on flight experience. We also provide brief reports of four accidents involving small Unmanned Aerial Systems (sUAS), most of which occurred during training. Inexperienced pilots should be limited to low-risk projects until they gain operational experience.

Stocking of pen-raised northern bobwhites (Colinus virginianus) into natural habitat is a common management strategy for this species, as is supplemental feeding of the cultivated seed milo (Sorghum bicolor) to wild bobwhites. However, milo may be deficient in minerals and/or other nutri- ents, leading to negative effects for bobwhites eating only milo. Additionally, pen-raised bobwhites with no experience eating seeds may be reluctant to eat native seeds they may find when released. We studied the effects of a milo-only diet on pen-raised bobwhites during the non-breeding season, and we tested the effects of mineral supplementation on bobwhites fed a milo diet. We also studied use and selection of native seeds by pen-raised bobwhites, and we tested the hypothesis that exposure to a cultivated seed (milo) diet improves the willingness of pen-raised bobwhites to eat native seeds.

The State Acres For wildlife Enhancement (SAFE) practice of the Conservation Reserve Program (CRP) in Tennessee is targeted to help

restore native habitats to benefit the northern bobwhite (Colinus virginianus) and other declining early successional wildlife. A survey of a subset of

participating landowners was conducted to assess landowner perceptions of and experiences with the practice and perceived wildlife response. The sur-

vey response rate was 58% (73 of 126 surveys mailed). All respondents were owners of the CRP SAFE tracts at the time of the survey, and most (91%)

managed the SAFE tracts themselves. SAFE contracts had been active for an average of seven years and ranged from 2 to 213 ha in size, with a mean

of 21.0±30.4 ha. Most of the respondents indicated they had received about the right amount of information prior to signing the SAFE contract (over

Management of northern bobwhite (Colinus virginianus) commonly focuses on creating cover and food for bobwhite throughout the year. Numerous studies have addressed these management practices and their impacts on bobwhite, but few have assessed the quantity of resources they pro- duce or the associated management cost per unit of production. My study assesses three common bobwhite habitat management practices (prescribed fire, roller chopping, and food plots) on the Cecil M. Webb-Babcock Wildlife Management Area in Charlotte County, Florida. I estimated production of the most common natural bobwhite forage (slough grass [Scleria spp.]) and the most common planted species (sesbania [Sesbania spp.]) in 80 wild- life exclosures located in areas that had been managed with prescribed fire only, in those that had been managed with both prescribed fire and roller chopping, and in food plots.

Black-bellied whistling-duck (Dendrocygna autumnalis; BBWD) is a neo-tropical species distributed in coastal areas of northern South Amer- ica, Central America, and southern North America. Despite their pervasiveness, the population distribution, survival, and harvest-mortality of BBWD in the southeastern United States remains unclear. We used BBWD sightings reported to eBird to delineate range expansion from 2006–2016 in Ala- bama, Florida, Georgia, Louisiana, Mississippi, South Carolina, and Texas. Concurrently, we used band-recovery data from 759 BBWD captured in five states (Georgia, Florida, South Carolina, Texas, and Louisiana) from 2014–2017 and Burnham live-dead models to calculate survival, recapture proba- bility, and annual recovery rate.

Waterfowl hunters participate in hunting for appreciative-, affiliative-, and achievement-oriented reasons. To investigate the influence of achievement-oriented factors on hunt quality, we analyzed post-hunt surveys completed by waterfowl hunters at four Mississippi Wildlife Management Areas (WMAs), 2008–2015. We used these questions to calculate a hunt quality score for each participant and tested whether variation in hunt quality was best explained by total number of ducks harvested, number of mallards harvested, total bag weight, or palatability of ducks. Hunt quality increased with total number of ducks and mallards harvested. Hunt quality scores increased with an increasing number of ducks harvested up to six total ducks (i.e., the daily allowable bag) and number of mallards up to 3 ducks (i.e., 1 less than the daily allowable bag during study). Our results indicate that harvesting ducks, especially mallards, is important to hunters at Mississippi WMAs.

Aerial waterfowl surveys are conducted on major wintering areas to provide regional population indices and determine habitat use of non-breeding waterfowl. Coastal Louisiana supports more than one quarter of the continental dabbling duck population during winter. Thus, consid- erable effort is allocated to monitoring waterfowl abundance in coastal Louisiana with implications for future waterfowl habitat management in the region. We conducted monthly surveys on nine state-owned coastal wildlife management areas and refuges, November–January 2004–2016. Across all sites and survey years, the most commonly observed species were gadwall (Mareca strepera), green-winged teal (Anas crecca), and mallard (Anas platy- rhynchos). Despite increases in continental breeding population indices to near record highs, their populations were stable region-wide in coastal Lou- isiana, with minor declines on some heavily-hunted and unmanaged areas.

Spatial variation in landscape composition can influence phenotypic expression in wildlife species and can improve management efforts to express certain phenotypic traits. We evaluated the influence of age, landscape composition, and physiographic province on white-tailed deer (Odo- coileus virginianus) antler characteristics using data from 16,622 male deer (age range: 1.5–3.5+ years old) harvested between 1997–2016 across five physiographic provinces in Georgia. Age and physiographic province influenced antler size index (ASI; P < 0.001). ASI of yearling males was greatest (x- = 53.37; SE = 0.39) in the Upper Coastal Plain and least (x- = 46.23; SE = 0.51) in the Lower Coastal Plain physiographic province. Given the differences in ASI among physiographic provinces, we evaluated how landscape composition within each physiographic province influenced ASI of 7,325 yearling (1.5-year-old) males.

Population surveys and removal efforts for wild pigs (Sus scrofa) have traditionally used a food-based attractant. However, some situations or locations where these activities may take place may not be conducive to the logistical challenges associated with storing or hauling large quantities of bait. Scent-based lures are lighter and easier to store than baits, and may negate some of these logistical challenges. Our goal was to examine the efficacy of a urine-based lure for attracting wild pigs to and retaining them at camera sites. We compared the initial arrival time and length of visits among boars, sounders, and juveniles at sites with a urine lure, whole corn, and a combination of the urine lure and whole corn during June and July 2017 on Lowndes Wildlife Management Area, Alabama. Our results indicated that sounders arrived at URINE sites quicker than other treatment sites, although we did not find these same results among boars or juveniles.

Though the eastern gray squirrel (Sciurus carolinensis) is an important game species throughout its range in North America, little is known about environmental factors that may affect survival. We investigated survival and predation of a hunted population of eastern gray squirrels on Lown- des Wildlife Management Area in central Alabama from July 2015–April 2017. This area experiences annual flooding conditions from November through the following September. Our Kaplan-Meier survival estimate at 365 days for all squirrels was 0.25 (0.14–0.44, 95% CL) which is within the range for previously studied eastern gray squirrel populations (0.20–0.58). There was no difference between male (0.13; 0.05–0.36, 95% CL) and female survival (0.37; 0.18–0.75, 95% CL, P=0.16). Survival was greatest in summer (1.00) and fall (0.65; 0.29–1.0, 95% CL) and lowest during winter (0.23; 0.11–0.50, 95% CL).

The eastern hellbender salamander (Cryptobranchus alleganiensis alleganiensis) is a protected species of concern in North Carolina as well as in several other states. Despite long-term efforts by the North Carolina Wildlife Resources Commission (NCWRC) and cooperating partners to improve understanding of hellbender status in the state, census of all known and potential populations is lacking. The species’ dependence upon clean, cold, well-oxygenated water restricts its distribution to North Carolina’s Blue Ridge Ecoregion, overlapping much of the state’s trout fishery. This overlap presented an opportunity for the NCWRC to educate trout anglers about hellbender conservation while also offering a chance to supplement existing data of the spatial and temporal distribution of the salamander by enlisting angler help.

The shoal bass (Micropterus cataractae) is a sport fish endemic to the Apalachicola-Chattahoochee-Flint Basin of the southeastern United States. Introgression with several non-native congeners poses a pertinent threat to shoal bass conservation, particularly in the altered habitats of the Chattahoochee River. Our primary objective was to characterize hybridization in shoal bass populations near Atlanta, Georgia, including a population inhabiting Big Creek and another in the main stem Chattahoochee River below Morgan Falls Dam (MFD). A secondary objective was to examine the accuracy of phenotypic identifications below MFD based on a simplified suite of characters examined in the field. Fish were genotyped with 16 micro- satellite DNA markers, and results demonstrated that at least four black bass species were involved in introgressive hybridization. Of 62 fish genotyped from Big Creek, 27% were pure shoal bass and 65% represented either F1 hybrids of shoal bass x smallmouth bass (M.

Lake Fork Reservoir, in northeast Texas, supports a nationally-recognized trophy largemouth bass (Micropterus salmoides, LMB) fishery which the Texas Parks and Wildlife Department (TPWD) has managed using restrictive harvest regulations since it was opened to public fishing in 1980. De- spite a long history of annual creel and electro fishing surveys conducted by TPWD, data on trophy fish is limited. Fisheries managers' inability to collect trophy-sized LMB using traditional sampling methods is probably the result of a combination of gear biases and low relative abundance of trophy-sized fish. We collected volunteer angler survey data on trophy-sized sh >3.18 kg, with interest in the sample above the upper bound of the protective slot- length limit (>609 mm TL), and evaluated the utility of this information to supplement creel and electro fishing survey data.

Geographical range of a species can be limited by environmental conditions such as temperature. This is important to understand when trying to establish a new fish population on the fringe of their range. The Oklahoma Department of Wildlife Conservation (ODWC) has been stocking tiger muskellunge (Esox masquinongy x E. lucius) in Lake Carl Etling in northwestern Oklahoma since 2014 with little success. This reservoir experiences a broad range of temperatures that could affect recruitment of tiger muskellunge, especially during times of the year when prey are not abundant. To explore the possible effects of temperature and prey availability on post-stocking survival, temperature tolerances of juvenile tiger muskellunge were determined in laboratory trials using starved and fed fish (n=9 each) acclimated to temperatures of 20, 24, and 28°C.

Saugeye are hatchery produced hybrids (female walleye [Sander vitreus] and male sauger [S. canadensis]) that have been introduced to aquatic systems throughout the United States. The Oklahoma Department of Wildlife Conservation (ODWC) stocks reservoirs throughout Oklahoma with saugeye to control overcrowded, stunted white crappie (Pomoxis annularis) populations and to provide recreational fishing opportunities. Because sacrificing fish regularly to remove otoliths for age estimation is often unpopular with anglers, a non-lethal means of obtaining age estimates for saugeye to describe population dynamics is desirable. Therefore, we compared age estimate precision between readers and structures (otoliths and dorsal spines), and compared age-based population parameters (growth, mortality, recruitment) derived from these age estimates. Structures were removed from 47 saugeye collected from Thunderbird Reservoir, Oklahoma via electro fishing.

In the 1970s and 1980s, blue catfish (Ictalurus furcatus) were introduced to the tidal rivers of Virginia. Current abundances and uncertainty about population characteristics of blue catfish generated concern for other economically important and imperiled species. We estimated natural mortality and size structure of blue catfish for four tidal river systems (i.e., James, Mattaponi, Pamunkey and Rappahannock). Using common empirical estimators with pooled data from the period 2002-2016, we calculated five estimates of natural mortality. Proportional size distributions were used to examine changes in size structure over time. Maximum observed age of 25 years indicated mature populations. Estimated mean instantaneous natural mortality (M) from five empirical estimators ranged from 0.13-0.19 in the four rivers. Temporal trends in size structure differed among rivers, likely due to differences in stocking timing and riverine productivity.

Blue catfish (Ictalurus furcatus) are native to the Coosa River drainage in northwest Georgia but have been widely introduced outside of this range including Lake Oconee, a 7677-ha impoundment on the Oconee River in central Georgia. Blue catfish abundance and growth rates have increased dramatically since their introduction in Lake Oconee, but their food habits are unknown. Therefore, food habits of blue catfish in this impoundment were determined by examining the stomachs of 808 specimens in the reservoir's upper and lower regions across all seasons from summer 2012 to summer 2013. Diet was summarized using the Relative Importance of specific prey by weight. In the upper region of the reservoir, Asian clams (Corbicula fluminea) were the dominant prey item during the summer (75.7%), fall (66.4%), and winter (37.6%); whereas crappie (Pomoxis spp.) was the dominant prey item in the spring (38.7%).

The New River, Virginia, supports a trotline fishery for catfish (Ictaluridae) that coexists with popular recreational fisheries for smallmouth bass (Micropterus dolomieu), muskellunge (Esox masquinongy), and walleye (Sander vitreus), yet no studies have examined trotline catches or bycatch of these game fish. Trotline effort was estimated by conducting off-site interviews of trotline fishers and field counts of active trotlines. Catch of catfish and bycatch were estimated with experimental trotline sets that used circle or J hooks and two bait types (i.e., live or cut bait). Catch averaged 12.1 catfish 100 hook nights-1. Experimental trotline sets baited with live bait fish captured predominantly channel catfish (Ictalurus punctatus) and flathead catfish (Pylodictis olivaris) but caught few smallmouth bass, muskellunge, or walleye. Cutbait caught fewer catfish, particularly flathead catfish, and fewer non-catfish species than live bait.

Stress is unavoidable in aquaculture and hence strains of fish that are resilient and adaptable to stress need to be developed. In teleosts, cortisol is considered the primary stress hormone and often increases in cortisol concentration correspond to a stress response. The objective of this study was to assess if cortisol responsiveness to stress in channel catfish (Ictalurus punctatus) influences susceptibility to Enteric Septicemia of Catfish (ESC) caused by Edwardsiella ictaluri under controlled conditions. Juvenile channel catfish were subjected to standardized hypoxia stress (1.8 mg L-1 of dissolved oxygen) to classify them as either low responders (LR) or high responders (HR) based on their plasma cortisol concentration. Fish in both groups were held either in individual or co-cultured in 80-L aquaria and were challenged with virulent E. ictaluri by an in situ bath immersion to evaluate their susceptibility to the pathogen.

Angling participation has stagnated or declined in many regions, threatening the political and financial support for fisheries management. Angler recruitment programs aim to counteract these trends, but most are public programs targeting public water bodies. There are about 4.5 million small ponds and lakes in the United States, most of which are privately owned. These systems may play a major yet hidden role in angler recruitment. Using an online survey of avid pond owners and managers, we explored the ideas that private waters are providing youth angling opportunities, increasing fishing participation, and contributing to angler recruitment. Survey results indicated that pond owners are engaged in angler recruitment and retention by providing youth fishing opportunities to friends and family beyond that generally available in traditional recruiting events on public waters.

A new tool to provide wetland services is the floating streambed wetland (FSW), an active hydroponic system consisting of a polymer matrix floating substrate in which living plants are established. Water is circulated from beneath the FSW and across a streambed on the upper FSW surface, coming into contact with biofilms attached to the polymer matrix and associated root structures. Research has shown that FSW technology is efficient in removing nutrients and water contaminants, and recent manufacturer reports claim that FSW technology may also increase total fish biomass in small water bodies. We evaluated this claim using a replicated small (526 m2) pond experiment and FSWs that covered 2.3% of pond surface area.

Earthen hatchery pond sediments may provide additional nutrients to the water column and act as a sink for nutrients and excess fertilizer. In 2008 and again in 2013 soil nutrient concentrations and phosphorus (P) adsorption were examined in five earthen ponds at Minor Clark Fish Hatchery, Kentucky. These ponds are annually filled with oligotrophic water from an upstream reservoir, fertilized, and drained during normal hatchery operations. Over time, pond nitrogen (N) and P additions have not kept up with soil losses. Soil extractable P concentrations decreased about an order of magnitude between 2008 and 2013. Soil %N decreased by nearly 50% over the same time period. Other soil nutrients (organic carbon, calcium, and manganese) remained the same. Soil collected in 2008 desorbed P under normal fish rearing water quality conditions.

Resource selection by female white-tailed deer (Odocoileus virginianus) and their offspring during the fawning season can influence survival and recruitment. The selection process in females is thought to represent the balancing of often competing demands to minimize predation risk and maximize resource availability to support the energetic demands of lactation. We used a distance-based approach to examine selection of fawn-rearing areas and locations within fawning areas for 20 radio-instrumented female white-tailed deer on Tensas River National Wildlife Refuge, Louisiana. We also examined selection of vegetative attributes at parturition sites (n = 20) and fawn bed sites (n = 106). Females selected fawn-rearing areas nearer to agriculture than expected given their home ranges, but within their fawn-rearing areas, females were located farther from agriculture and reforestation than expected.

White-tailed deer (Odocoileus virginianus) populations located at the mouth of the Mississippi River are of historical significance as they have been a major source for restocking in Louisiana since the 1960s. Apparent population declines of these deer since the 1990s led the Louisiana Department of Wildlife and Fisheries to initiate a study to gather demographic data on white-tailed deer on the 46,540-ha Pass-a-Loutre Wildlife Management Area (PALWMA). We captured and individually marked 57 deer on PALWMA from 2007 to 2012. We monitored travel corridors using un-baited trail cameras and recorded all sightings of marked and unmarked individuals until 2014. We collected 4,325 photographic observations of white-tailed deer with 340 (8%) resightings of individually marked deer. Estimated apparent annual survival for female and male white-tailed deer was 0.48 ± 0.07 and 0.78 ± 0.06, respectively.

White-tailed deer (Odocoileus virginanus) are the most sought after game species in Kentucky. Deer numbers in southeast Kentucky are relatively low compared to other areas of the state, even after a decade of restrictive doe harvest and prior population supplementation. We estimated survival and assessed cause-specific mortality of a representative deer population in this low-density area within or near the Redbird District of the Daniel Boone National Forest in southeastern Kentucky from January 2014-January 2017. Estimated annual survival for does averaged 0.89 and was relatively high compared to similar studies. Deer-vehicle collisions and poaching caused 13 of 18 (72%) deaths. We recommend longer-term studies at these and other sites in this area to better understand deer population dynamics and their relationship to important habitat components so as to inform regional management of this important game species.

Most state wildlife agencies collect harvest data to inform management decisions. However, these data are typically considered across relatively short time periods and are rarely revisited. We present a case study using historical records to investigate potential agents (i.e., harvest, predation, and forest change) influencing the declining white-tailed deer (Odocoileus virginianus) population in the north Georgia mountains. We used long-term black bear (Ursus americanus) and deer harvest data, and indices of forest stand conditions from 1979-2015 on eight Wildlife Management Areas (WMAs) in the north Georgia mountains. During 1979-2015, harvest of male and female deer declined by 85% and 97%, respectively. Over the same time period, mean yearling male deer body weight increased by 21%, mean antler diameter increased by 62%, and mean antler beam length increased by 92%.