As hunting participation decreases in the United States, wildlife agencies may consider increasing license fees or creating additional ones to sustain their programs. We assessed Willingness to Pay (WTP) for fall and spring eastern wild turkey (Meleagris gallopavo silvestris) hunting permits in Mississippi using the Contingent Valuation Method. Hunters were willing to pay more for a spring hunting permit than for a fall hunting permit and demand for spring hunting permits was also higher. Median WTP for fall and spring turkey hunting permits was US$11.00 and $36.25, respectively. Revenue can be maximized at $277,506 and $739,821 for the state by placing the permit fees at $20.62 and $39.75 for fall and spring hunting permits, respectively. These methods can be used to set fees and assess demand for a variety of recreational activities while maximizing participation.